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alkaline phosphatase treated pbluescript ii sk plasmid cloning vector  (New England Biolabs)


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    Structured Review

    New England Biolabs alkaline phosphatase treated pbluescript ii sk plasmid cloning vector
    Alkaline Phosphatase Treated Pbluescript Ii Sk Plasmid Cloning Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alkaline+phosphatase+treated+pbluescript+ii+sk+plasmid+cloning+vector/T4+DNA+Ligase/us07655425-173-14-37
    Average 99 stars, based on 33541 article reviews
    alkaline phosphatase treated pbluescript ii sk plasmid cloning vector - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Uses of a chemokine receptor able to bind to MCP-1, MIP-1α and/or RANTES
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK-plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Article Title: Chemokine receptor able to bind to MCP-1, MIP-1 α and/or rantes and its uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK-plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products were diluted to 100 μl with 1× TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Article Title: Substances and their uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK− plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products a were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Cloning:

    Article Title: Uses of a chemokine receptor able to bind to MCP-1, MIP-1α and/or RANTES
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK-plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Article Title: Chemokine receptor able to bind to MCP-1, MIP-1 α and/or rantes and its uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK-plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products were diluted to 100 μl with 1× TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Article Title: Substances and their uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK− plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products a were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Ligation:

    Article Title: Uses of a chemokine receptor able to bind to MCP-1, MIP-1α and/or RANTES
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK-plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Article Title: Chemokine receptor able to bind to MCP-1, MIP-1 α and/or rantes and its uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK-plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products were diluted to 100 μl with 1× TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Article Title: Substances and their uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK− plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products a were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..

    Plasmid Preparation:

    Article Title: Substances and their uses
    Article Snippet: .. Blunt-ended PCR product (10 ng) was ligated to 50 ng of Eco RV digested, alkaline phosphatase treated pBluescript II SK− plasmid cloning vector in a 20 μl volume using 2 μl of T4 DNA ligase (400 000 units/ml) (New England Biolabs) for at least 16 h at 15° C. Ligation products a were diluted to 100 μl with 1×TE (10 mM Tris-HCl pH 8.0/1 mM EDTA) and phenol/chloroform extracted as described previously. ..



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    99
    New England Biolabs alkaline phosphatase treated pbluescript ii sk plasmid cloning vector
    Alkaline Phosphatase Treated Pbluescript Ii Sk Plasmid Cloning Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alkaline+phosphatase+treated+pbluescript+ii+sk+plasmid+cloning+vector/T4+DNA+Ligase/us07655425-173-14-37
    Average 99 stars, based on 1 article reviews
    alkaline phosphatase treated pbluescript ii sk plasmid cloning vector - by Bioz Stars, 2026-10
    99/100 stars
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